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Livestock Production
Tuesday, April 18, 2006 11:05:26 AM
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Increase in AME of corn and corn-soybean meal by a multi-enzyme liquid formulation

A. Lim and H.M. Tan



RD20 contains a mixture of ß -amylase, ß -glucanase, protease, cellulase and xylanase and is targeted at feedmills using high pelleting temperatures.


This paper summarizes an in vivo trial that evaluated the effect of RD20 at 0.5 kg/tonne dosage, on the apparent metabolisable energy (AME) of corn and corn-soybean meal diets in 21-day old male Ross chicks.


The positive energy sparing effect on corn-soybean meal diet, which is largely attributed to the synergistic effect of the glycosylase and protease activities in RD20, represents a cost-saving opportunity in broiler feed.


The use of liquid enzymes in post-pellet application on broiler feed is fast becoming a trend in major feedmills due to the high processing temperature (>90ºC). Despite its clear advantage in delivering enzymes without the risk of thermal inactivation, this approach needs to overcome three hurdles:

    • Stability of enzymes in non-physiological liquid media is much lower.

    • Increased risk of proteolysis due to presence of protease and side protease activities.

    • High degree of precision in applicators to ensure homogeneity of feed enzymes in the feed.

Kemin Industries has developed RD20 to improve the nutritional value of corn-soybean diets. This formulation differs from most commercial liquid enzyme products in that it contains a significant amount of protease, which has been shown to improve nutritional value of diets containing soybean meal.


The positive energy saving effects of RD20 in broiler chicks fed on corn and corn-soybean meal diets are presented in this paper.


Two random samples from a one kg composite feed sample were assayed for ß -glucanase activity using a plate diffusion assay. ß -Glucanase activity in the feed samples was calculated from a calibration curve using RD20 at different dosages.


A total of 220 day-old male broiler chicks (Ross) were obtained from a local hatchery and reared in floor pens on commercial starter/ grower diets. At 21 days of age, the birds were transferred to colony cages that had facilities for total collection of excreta.


The corn-soybean meal diet was formulated to an ME of 13.39 MJ/kg (normal ME).


The Reduced ME diet was created by reducing the amount of soya oil from 53 g/kg to 30 g/kg.


Both the corn-soybean meal diets (normal and reduced ME) were cold-pelleted (70ºC), while the corn diets were fed in mash form.


RD20 was diluted at one part enzyme to 20 parts water and sprayed while the diets were being mixed in a horizontal mixer. Diets were offered ad libitum and water was available at all times.


On day 25, birds were weighed individually and birds with relatively high or low body weights were discarded. A total of 180 birds were chosen and distributed into 36 groups (pens) of five birds each so that average weights per pen was nearly equal.


Each of the six dietary treatments were then randomly assigned to six pens (six replicates per treatment).


The AME values were determined using a classical total collection method. The birds were fed the treatment diets for seven days (from day 25) with the first three days serving as an adaptation period. During the last four days, feed intake was monitored, and the excreta were collected daily, weighed and pooled within a pen. Pooled excreta were mixed well into slurry and two samples per pen were obtained and freeze-dried. Dried excreta samples were ground to pass through a 0.5 mm sieve and stored in airtight plastic containers at - 40ºC for chemical analyses.


The gross energy (GE) of the diets and excreta samples was determined using an adiabatic bomb calorimeter.



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Article made possible through the contribution of the Australian Poultry Science Symposium (APSS) 2006.

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