Factors associated with the occurrence of MRSA CC398 in herds of fattening pigs in Germany
The purpose of this study was to investigate the prevalence of MRSA in herds of fattening pigs in different regions of Germany, and to determine factors associated with the occurrence of this pathogen. For this purpose pooled dust samples were collected, and a questionnaire covered information regarding herd characteristics and management practices. Samples were pre-enriched in high-salt medium followed by selective enrichment containing cefoxitin/aztreonam, and culturing. Presumptive colonies were confirmed by multiplex-PCR targeting nuc-, mecA- and 16S rRNA-genes. Isolates were spa- and SCCmec-, and in selected cases, multilocus sequence-typed. Susceptibilities to 13 antimicrobials were determined by broth microdilution. Statistical analysis was carried out using backward stepwise logistic regression to calculate odds ratios with the MRSA test result as the outcome and herd characteristics as categorical covariates.
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Article made possible through the contribution of Katja Alt, Alexandra Fetsch, Andreas Schroeter, Beatriz Guerra, Jens A Hammerl, Stefan Hertwig, Natalja Senkov, Anna Geinets, Christine Mueller-Graf, Juliane Braeunig, Annemarie Kaesbohrer, Bernd Appel, Andreas Hensel, Bernd-Alois Tenhagen and BioMed Central.