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Functional Additives
Tuesday, April 17, 2012 2:44:53 PM
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Intestinal infection following aerosol challenge of calves with Mycobacterium avium subspecies paratuberculosis

 
Susanne WF Eisenberg, Ad P Koets, Mirjam Nielen, Dick Heederik, Rienske Mortier, Jeroen De Buck and Karin Orsel
 
 

A challenge experiment was performed to investigate whether administration of Mycobacterium avium subsp. paratuberculosis (MAP) via the respiratory route leads to MAP infection in calves. Eighteen calves from test negative dams were randomly allocated to four groups. Six calves were challenged with MAP nasally and six calves were challenged by transtracheal injection; three orally challenged calves served as positive controls, and three non challenged calves as negative controls.

 

The challenge was performed as a nine-fold trickle dose, 107 CFU in total. Blood and faecal samples were collected frequently. Calves were euthanized three months post-challenge and extensively sampled. Blood samples were tested for the presence of antibodies and interferon gamma producing cells by ELISA. Faecal and tissue samples were cultured in liquid culture system and the presence of MAP was confirmed by IS900 realtime PCR. 

           
           

For the full article, please click here.

 

Article made possible through the contribution of Susanne WF Eisenberg, Ad P Koets, Mirjam Nielen, Dick Heederik, Rienske Mortier, Jeroen De Buck, Karin Orsel and BioMed Central.

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